NA11438
DNA from Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
DNA from Somatic cell hybrid
|
|
Confirmation
|
Karyotypic analysis and In situ hybridization
|
|
ISCN
|
Human/Chinese hamster hybrid retaining human der(5)t(5;13) in 96% of cells & #11 (100%), 18 (88%), & 20 (56%)
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1p36, PND. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3, D5S10, and gave a positive result with a probe for 5q33q35, PDGFRB. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 5p15.3-p15.1, D5S208, and gave positive results with primers for 5p14-p12, C9, 5q11.2-q13.1, D5S351, 5q13.3-q14, HMGCR, 5q32, D5S372, and 5q34-q35, GABRA1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6pterp24, D6S7. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 11q23.3, ETS1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 13q34, D13S3. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 13q11-q12, D13S232, 13q14.3, RB1, 13q22-q31, D13S317, and 13q34, F7.1. |
| |
| Remarks |
Line HHW805; human/Chinese hamster hybrid; retains human der(5)t(5;13)(5qter> 5p15.2::13p12>13qter) in 96% of cells & other human chromosomes; grown in medium containing 0.2mM proline at 39C |
|
|