NA11753
DNA from Somatic cell hybrid
Description:
CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 7 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Sample Source
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DNA from Somatic cell hybrid
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/hamster somatic cell hybrid retaining a human der(3)t(3;7)(p21.1;p13) in 56% of cells and #4, 8, 19, 21, & Y
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line 3;7/UC2-1; produced by fusion of cells from pt with Greig polysyndactyly syn, t(3;7)(p21.1;p13), with CHO Urd-C cells; grown in uridine-def F12 medium with 5% dialyzed FBS; retains hum der(3) t(3;7) in 56% of cells & other hu chroms |
| Ayyanathan K, Naylor SL, Kunapuli SP, Structural characterization and fine chromosomal mapping of the human P2Y1 purinergic receptor gene (P2RY1). Somat Cell Mol Genet22:419-24 1996 |
| PubMed ID: 9039850 |
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| Naylor SL, Buys CH, Carritt B, Report and abstracts of the Fourth International Workshop on Human Chromosome 3 Mapping Cytogenetics and cell genetics65:2-50 1994 |
| PubMed ID: 8104766 |
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| Drabkin H, Wright M, Jonsen M, Varkony T, Jones C, Sage M, Gold S, Morse H, Mendez M, Erickson P, Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3. Genomics8:435-46 1990 |
| PubMed ID: 2286371 |
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| Drabkin H, Sage M, Helms C, Green P, Gemmill R, Smith D, Erickson P, Hart I, Ferguson-Smith A, Ruddle F, et al, Regional and physical mapping studies characterizing the Greig polysyndactyly 3;7 chromosome translocation, t(3;7)(p21.1;p13). Genomics4:518-29 1989 |
| PubMed ID: 2545596 |
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