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NA11936 DNA from Somatic cell hybrid

Description:

CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Quantity 0.050mg
Quantitation Method Please see our FAQ
Cell Type Somatic cell hybrid
Transformant Untransformed
Sample Source DNA from Somatic cell hybrid
Confirmation Clinical summary/Case history
ISCN Mouse/human hybrid retaining human der(16)t(11;16)(q13;p11)
Remarks Line CF52-46-6; produced by the fusion of human fibroblast, GM03005, 46,XX,t(11;16) (q13;p11), with HPRT-deficient mouse A9 cells; grown in medium with adenine, aminopterin, and thymidine; retains human der(16)t(11;16) in 100% of cells

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 4p16.2p15.1, D4S20.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11p15.5, D11S12, and gave a positive result with a probe for 11q23.3, ETS1. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 11p13, CAT, and gave a positive result with a primer for 11q21-q22, CLG.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 12q14q24.1, D12S7.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 Dna from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 14q32.1q32.3, D14S13.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 16q13q22.1, MT2, and gave a negative result with a probe for 16pterp13, D16S36. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 16q22.1, LCAT, 16q23.2, D16S266, 16q24.3, PRSM1, and gave a negative result with a primer for 16p13.3, HBA.
 

Phenotypic Data

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Remarks Line CF52-46-6; produced by the fusion of human fibroblast, GM03005, 46,XX,t(11;16) (q13;p11), with HPRT-deficient mouse A9 cells; grown in medium with adenine, aminopterin, and thymidine; retains human der(16)t(11;16) in 100% of cells

Publications

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O'Keefe DS, Bacich DJ, Heston WD, Comparative analysis of prostate-specific membrane antigen (PSMA) versus a prostate-specific membrane antigen-like gene The Prostate58:200-10 2004
PubMed ID: 14716746
 
O'Keefe DS, Su SL, Bacich DJ, Horiguchi Y, Luo Y, Powell CT, Zandvliet D, Russell PJ, Molloy PL, Nowak NJ, Shows TB, Mullins C, Vonder Haar RA, Fair WR, Heston WDW, Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene [In Process Citation] Biochim Biophys Acta1443:113-27 1998
PubMed ID: 9838072
 
Leek J, Lench N, Maraj B, Bailey A, Carr IM, Andersen S, Cross J, Whelan P, MacLennan KA, Meredith DM, Prostate-specific membrane antigen: evidence for the existence of a second related human gene British journal of cancer72:583-8 1995
PubMed ID: 7669565
 
Junien C, van Heyningen V, Evans G, Little P, Mannens M, Report of the second chromosome 11 workshop. Genomics12:620-5 1992
PubMed ID: 1348491
 
Julier C, Nakamura Y, Lathrop M, O'Connell P, Leppert M, Litt M, Mohandas T, Lalouel JM, White R, A detailed genetic map of the long arm of chromosome 11. Genomics7:335-45 1990
PubMed ID: 1973139
 
Koeffler HP, Sparkes RS, Stang H, Mohandas T, Regional assignment of genes for human alpha-globin and phosphoglycollate phosphatase to the short arm of chromosome 16. Proc Natl Acad Sci U S A78:7015-8 1981
PubMed ID: 6273902

External Links

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dbSNP dbSNP ID: 11657
Pricing
International/Commercial/For-profit:
$449.00USD
U.S. Academic/Non-profit/Government:
$263.00USD
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