NA11938
DNA from Somatic cell hybrid
Description:
CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Sample Source
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DNA from Somatic cell hybrid
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Confirmation
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Clinical summary/Case history
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ISCN
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Mouse/human hybrid retaining human der(11)t(11;18) & #3, 5, 10, 13, 14, 15, 17, 19, 20, 22, & X
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 4p16.2p15.1, D4S20. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3, D5S10. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 9p22, IFNB, and 9q34.1, ABL. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11p15.5, D11S12, and gave positive results with probes for 11q23.3, ETS1, and 11p13, CAT. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16q13q22.1, MT2, and 16pterp13, D16S36. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 18q12.3, D18S6, and 18q23, MBP. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 18p11.31-p11.22, TYMS, and gave negative results with a primer for 18p11.1-q11.2, D18S44, and a different primer for the same locus. |
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| Remarks |
Line PEL16; produced by the fusion of human lymphoblasts, 46,XY,t(11;18) (p15.4;p11.1), with HPRT-deficient mouse myeloma SP2/O-Ag14 cells; grown without selection; retains human der(11)t(11;18) in 92% of cells & other human chromos |
| Schwienbacher C, Sabbioni S, Barbanti-Brodano G, Negrini M, Refined subchromosomal location of 21 expressed sequence tags from unknown genes at region 11p15. Eur J Hum Genet5:214-7 1997 |
| PubMed ID: 9359042 |
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| van Kessel AG, Straub RE, Silverman GA, Gerken S, Overhauser J, Report and abstracts of the second international workshop on human chromosome 18 mapping. Doorwerth, the Netherlands, July 19-20, 1993. Cytogenet Cell Genet65:142-65 1994 |
| PubMed ID: 8222751 |
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| Junien C, van Heyningen V, Evans G, Little P, Mannens M, Report of the second chromosome 11 workshop. Genomics12:620-5 1992 |
| PubMed ID: 1348491 |
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| Gessler M, Hameister H, Henry I, Junien C, Braun T, Arnold HH, The human MyoD1 (MYF3) gene maps on the short arm of chromosome 11 but is not associated with the WAGR locus or the region for the Beckwith- Wiedemann syndrome. Hum Genet86:135-8 1990 |
| PubMed ID: 2176177 |
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| Couillin P, Azoulay M, Henry I, Ravise N, Grisard MC, Jeanpierre C, Barichard F, Metezeau P, Candelier JJ, Lewis W, et al, Characterization of a panel of somatic cell hybrids for subregional mapping along 11p and within band 11p13. Subdivision of the WAGR complex region. Hum Genet82:171-8 1989 |
| PubMed ID: 2722195 |
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| Henry I, Jeanpierre M, Couillin P, Barichard F, Serre JL, Journel H, Lamouroux A, Turleau C, de Grouchy J, Junien C, Molecular definition of the 11p15.5 region involved in Beckwith- Wiedemann syndrome and probably in predisposition to adrenocortical carcinoma. Hum Genet81:273-7 1989 |
| PubMed ID: 2921038 |
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