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NA11944 DNA from Somatic cell hybrid

Description:

CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Quantity 0.050mg
Quantitation Method Please see our FAQ
Cell Type Somatic cell hybrid
Transformant Untransformed
Sample Source DNA from Somatic cell hybrid
Confirmation Clinical summary/Case history
ISCN Chinese hamster/human hybrid retaining human 11pter>cen translocated to a hamster chromosome
Remarks Line J1-11; produced by the fusion of normal human amniotic fluid cells with Chinese hamster gly-A auxotrophic mutant cells; grown without selection; retains human 11pter>cent translocated to a hamster chromosome in 68% of cells

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 4p16.2p15.1, D4S20.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 11p15.5, D11S12, and gave a negative result with a probe for 11q23.3, ETS1. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 11p13, CAT, and gave a negative result with a primer for 11q21-q22, CLG.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16q13q22.1, MT2, and 16pterp13, D16S36.
 

Phenotypic Data

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Remarks Line J1-11; produced by the fusion of normal human amniotic fluid cells with Chinese hamster gly-A auxotrophic mutant cells; grown without selection; retains human 11pter>cent translocated to a hamster chromosome in 68% of cells

Publications

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O'Keefe DS, Bacich DJ, Heston WD, Comparative analysis of prostate-specific membrane antigen (PSMA) versus a prostate-specific membrane antigen-like gene The Prostate58:200-10 2004
PubMed ID: 14716746
 
Prawitt D, Enklaar T, Klemm G, Gartner B, Spangenberg C, Winterpacht A, Higgins M, Pelletier J, Zabel B, Identification and characterization of MTR1, a novel gene with homology to melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical region on chromosome 11p15.5 and showing allele-specific expression. Curr Biol9(2):203-16 2000
PubMed ID: 10607831
 
Gabriel JM, Higgins MJ, Gebuhr TC, Shows TB, Saitoh S, Nicholls RD, A model system to study genomic imprinting of human genes. Proc Natl Acad Sci U S A95:14857-62 1998
PubMed ID: 9843980
 
O'Keefe DS, Su SL, Bacich DJ, Horiguchi Y, Luo Y, Powell CT, Zandvliet D, Russell PJ, Molloy PL, Nowak NJ, Shows TB, Mullins C, Vonder Haar RA, Fair WR, Heston WDW, Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene [In Process Citation] Biochim Biophys Acta1443:113-27 1998
PubMed ID: 9838072
 
Leek J, Lench N, Maraj B, Bailey A, Carr IM, Andersen S, Cross J, Whelan P, MacLennan KA, Meredith DM, Prostate-specific membrane antigen: evidence for the existence of a second related human gene British journal of cancer72:583-8 1995
PubMed ID: 7669565
 
Tokino T, Takahashi E, Mori M, Tanigami A, Glaser T, Park JW, Jones C, Hori T, Nakamura Y, Isolation and mapping of 62 new RFLP markers on human chromosome 11. Am J Hum Genet48:258-68 1991
PubMed ID: 1671318
 
Glaser T, Housman D, Lewis WH, Gerhard D, Jones C, A fine-structure deletion map of human chromosome 11p: analysis of J1 series hybrids. Somat Cell Mol Genet15:477-501 1989
PubMed ID: 2595451

External Links

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dbSNP dbSNP ID: 11663
Pricing
International/Commercial/For-profit:
$449.00USD
U.S. Academic/Non-profit/Government:
$263.00USD
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