NA12448
DNA from Somatic cell hybrid
Description:
CHROMOSOME 9 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Sample Source
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DNA from Somatic cell hybrid
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a human der(9)t(9;17) in 96% of cells, #5 (84%), & 22 (100%)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 9p22, IFNB, and 9q34.1, ABL. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9pter-p22, D9S178, and 9p24, WI-7297, and gave positive results with primers for 9p21, D9S270, 9p22, IFNA, 9q22.3q31, ALDOB, 9q34.3, DBH, and 9q34-qter, D9S158 |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2, and gave a positive result with a probe for 17q21.3q23, MPO. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 17q25-qter, D17S784. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 18q12.3, D18S6, and 18q23, MBP. |
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| Remarks |
Line UMHG-17/2; produced by the fusion of human cells, 46,XY,t(9;17)(p22;q22), with TK-deficient Chinese hamster a23 cells; selected in HAT medium; retains human der(9)t(9;17) in 96% of cells & other human chromosomes |
| Hong H, Xu L, Liu J, Jones WD, Su Z, Ning B, Perkins R, Ge W, Miclaus K, Zhang L, Park K, Green B, Han T, Fang H, Lambert CG, Vega SC, Lin SM, Jafari N, Czika W, Wolfinger RD, Goodsaid F, Tong W, Shi L, Technical reproducibility of genotyping SNP arrays used in genome-wide association studies PloS one7:e44483 2012 |
| PubMed ID: 22970228 |
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| Wong A, Vallender EJ, Heretis K, Ilkin Y, Lahn BT, Martin CL, Ledbetter DH, Diverse fates of paralogs following segmental duplication of telomeric genes Genomics84:239-47 2004 |
| PubMed ID: 15233989 |
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| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
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| Flejter WL, Watkins M, Abel KJ, Chandrasekharappa SC, Weber BL, Collins FS, Glover TW, Isolation and characterization of somatic cell hybrids with breakpoints spanning 17q22-->q24. Cytogenet Cell Genet64:222-3 1993 |
| PubMed ID: 8404043 |
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