NA14198
DNA from Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Sample Source
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DNA from Somatic cell hybrid
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a der(5)t(5;13)(p15.2;p11) in 100% of cells, #4 (88%), 8 (96%), & 18 (96%)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 5p14-p12, C9, 5q13.3-q14, HMGCR, 5q34-q35, GABRA1, and 5q21-q22, MCC, and gave a negative result with a primer for 5p15.3-p15.1, D5S208. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 13q11-q12, D13S232, 13q14.3, RB1, and 13q22q31, D13S317. |
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| Remarks |
Line HHW792; produced by the fusion of human cells with Chinese hamster cells; grown in medium with 0.2mM proline at 39C; retains a human der(5)t(5;13) (p15.2;q11) in ??% of cells & other human chromosomes |
| Overhauser J, Huang X, Gersh M, Wilson W, McMahon J, Bengtsson U, Rojas K, Meyer M, Wasmuth JJ, Molecular and phenotypic mapping of the short arm of chromosome 5: sublocalization of the critical region for the cri-du-chat syndrome. Hum Mol Genet3:247-52 1994 |
| PubMed ID: 8004090 |
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