IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, and Xq21.3, DXS3. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2p13, TGFA. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3q13.1-q13.2, D3S1310, 3q26.2-q27, GLUT2, and 3q28-q29, D3S1311. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 5p14-p12, C9, 5q13.3-q14, HMGCR, and 5q34-q35, GABRA1. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7pter-p22, D7S472, and 7p12, EGFR, and gave a positive result with a primer for 7q36-qter, D7S550. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 15q21-q22.2, B2M. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 18p11.1-q11.2, D18S44, and 18q12.1, TTR, and gave negative results with primers for 18p11.31-p11.22, TYMS, and 18q23, D18S1390. |
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