NA14560
DNA from Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
DNA from Somatic cell hybrid
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
Human/Chinese hamster hybrid retaining a human del(18)(p11.21) in 100% of cells and other human chromosomes
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 18p11.1-p11.2, D18S44, 18q12.1, TTR, and 18q23, D18S1390, and gave a negative result with a primer for 18p11.31p11.22, TYMS. |
| |
| Remarks |
Line JH252; produced by the fusion of human lymphoblasts, 46,XX,del(18p11), with Chinese hamster UCW206 cells; grown at 39C; 100% of cells retain a human del(18)(p11.21) and other human chromosomes |
| Kline AD, Rojas K, Mewar R, Moshinsky D, Overhauser J, Somatic cell hybrid deletion map of human chromosome 18. Genomics13:1-6 1992 |
| PubMed ID: 1577474 |
|
|