NA14561
DNA from Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
DNA from Somatic cell hybrid
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/Chinese hamster hybrid retaining a human del(18)(q21.31) in 92% of cells
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18q23, D18S1390, and gave positive results with primers for 18p11.31-p11.22, TYMS, and 18q12.1, TTR. |
| |
| Remarks |
Line JH254; produced by the fusion of human lymphoblasts, del(18)(pter> q21.31:), with Chinese hamster UCW206 cells; grown at 39C; 92% of cells retain a human del(18)(q21.31) |
| Mahr RN, Moberg PJ, Overhauser J, Strathdee G, Kamholz J, Loevner LA, Campbell H, Zackai EH, Reber ME, Mozley DP, Brown L, Turetsky BI, Shapiro RM, Neuropsychiatry of 18q- syndrome. Am J Med Genet67(2):172-8 1996 |
| PubMed ID: 8723044 |
| |
| Strathdee G, Zackai EH, Shapiro R, Kamholz J, Overhauser J, Analysis of clinical variation seen in patients with 18q terminal deletions. Am J Med Genet59(4):476-83 1995 |
| PubMed ID: 8585568 |
| |
| Rojas K, Overhauser J, Sublocalization of 21 chromosome 18-specific microsatellite markers. Genomics18:169-71 1993 |
| PubMed ID: 8276412 |
|
|