NA14599

NA14599 DNA from Somatic cell hybrid

Description:

CHROMOSOME 6 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

Overview

Repository

NIGMS Human Genetic Cell Repository

Subcollection

Hybrids

Quantity

0.050mg

Quantitation Method

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Cell Type

Somatic cell hybrid

Transformant

Untransformed

Sample Source

DNA from Somatic cell hybrid

Confirmation

Clinical summary/Case history

ISCN

Human/Chinese hamster hybrid retaining a human del(6)(q21q25) in 84% of cells & a human Y (96%)

Remarks

Clone of line R21-1B; human/chinese hamster ovary somatic cell hybrid retaining a human del(6)(q21q25) in 80% of cells and a human Y in 88%; grown in medium without selection

Characterizations

IDENTIFICATION OF SPECIES OF ORIGIN

Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis

GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6

PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 6q21-q23.3, D6S292, and gave positive results with primers for 6q27-qter, D6S297, 6q24-q27, MAS1, 6p21.3, TNFA, and 6p25, D6S344.

Phenotypic Data

Remarks

Clone of line R21-1B; human/chinese hamster ovary somatic cell hybrid retaining a human del(6)(q21q25) in 80% of cells and a human Y in 88%; grown in medium without selection

External Links

dbSNP

dbSNP ID: 12128

Pricing

Commercial/For-profit:

$359.00USD

Academic/Non-profit/Government:

$214.00USD