AG05337
Epithelial from Retina of eye
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Retina of eye
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Cell Type
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Epithelial
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Tissue Type
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Retina, pigmented
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Transformant
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Untransformed
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Sample Source
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Epithelial from Retina of eye
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
22 |
| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Retinal pigmented epithelial line RPE-28 was initiated using explants of retinal tissue from a fetus of 13 cm crown-rump length obtained 10/13/81. The cell morphology is epithelial-like. The karyotpye is 46,XY; normal diploid male. An SV40-transformed cell line from this same donor is AG06096A. |
| Pignolo RJ, Cristofalo VJ, Rotenberg MO, Senescent WI-38 cells fail to express EPC-1, a gene induced in young cells upon entry into the G0 state. J Biol Chem268:8949-57 1993 |
| PubMed ID: 8473338 |
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| Gupta RS, Dudani AK, Species-specific differences in the toxicity of rhodamine 123 towards cultured mammalian cells. J Cell Physiol130:321-7 1987 |
| PubMed ID: 3558490 |
| Cumulative PDL at Freeze |
22 |
| Passage Frozen |
6 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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