NG05337
DNA from Epithelial
Description:
APPARENTLY HEALTHY FETAL TISSUE
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Apparently Healthy Collection |
| Quantity |
10 µg |
| Quantitation Method |
Please see our FAQ |
|
Biopsy Source
|
Retina of eye
|
|
Cell Type
|
Epithelial
|
|
Tissue Type
|
Retina, pigmented
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
DNA from Epithelial
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Freeze |
22 |
| Passage Frozen |
6 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Retinal pigmented epithelial line RPE-28 was initiated using explants of retinal tissue from a fetus of 13 cm crown-rump length obtained 10/13/81. The cell morphology is epithelial-like. The karyotpye is 46,XY; normal diploid male. An SV40-transformed cell line from this same donor is AG06096A. |
| Pignolo RJ, Cristofalo VJ, Rotenberg MO, Senescent WI-38 cells fail to express EPC-1, a gene induced in young cells upon entry into the G0 state. J Biol Chem268:8949-57 1993 |
| PubMed ID: 8473338 |
| |
| Gupta RS, Dudani AK, Species-specific differences in the toxicity of rhodamine 123 towards cultured mammalian cells. J Cell Physiol130:321-7 1987 |
| PubMed ID: 3558490 |
|
|