AG10788
Fibroblast from Skin, Thigh
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thigh
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
7.23 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor had autopsy confirmed Alzheimer's disease with an onset of symptoms after age 70. Donor's parent & 3 siblings are also affected. The donor was the product of a consanguineous marriage. The biopsy was taken ante-mortem from the skin of the right thigh and the culture was initiated on 12/26/89 using explants of minced tissue. The cell morphology is fibroblast-like. Culture is frozen at PDL 4. The karyotype is normal with 6% of the cells examined showing random chromosome loss/gain and 12% showing random chromosomal rearrangements. Donor's affected siblings are AG10984A Lymphoblast, AG11001 Lymphoblast, and AG10987 Lymphoblast. A lymphoblast culture from same donor is AG10956B. Donor was homozygous for the 4 allele of apolipoprotein E. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Lee H, Cho S, Kim MJ, Park YJ, Cho E, Jo YS, Kim YS, Lee JY, Thoudam T, Woo SH, Lee SI, Jeon J, Lee YS, Suh BC, Yoon JH, Go Y, Lee IK, Seo J, ApoE4-dependent lysosomal cholesterol accumulation impairs mitochondrial homeostasis and oxidative phosphorylation in human astrocytes Cell reports42:113183 2022 |
| PubMed ID: 37777962 |
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| Iannuzzi F, Frisardi V, Annunziato L, Matrone C, Might Fibroblasts from Patients with Alzheimer's Disease Reflect the Brain Pathology? A Focus on the Increased Phosphorylation of Amyloid Precursor Protein Tyr Brain sciences11:113183 2020 |
| PubMed ID: 33466666 |
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| Joshi AU, Van Wassenhove LD, Logas KR, Minhas PS, Andreasson KI, Weinberg KI, Chen CH, Mochly-Rosen D, Aldehyde dehydrogenase 2 activity and aldehydic load contribute to neuroinflammation and Alzheimer's disease related pathology Acta neuropathologica communications7:190 2019 |
| PubMed ID: 31829281 |
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| Meyer K, Feldman HM, Lu T, Drake D, Lim ET, Ling KH, Bishop NA, Pan Y, Seo J, Lin YT, Su SC, Church GM, Tsai LH, Yankner BA, REST and Neural Gene Network Dysregulation in iPSC Models of Alzheimer's Disease Cell reports26:1112-1127.e9 2018 |
| PubMed ID: 30699343 |
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| Khan TK, Alkon DL, An internally controlled peripheral biomarker for Alzheimer's disease: Erk1 and Erk2 responses to the inflammatory signal bradykinin Proceedings of the National Academy of Sciences of the United States of America103:13203-7 2006 |
| PubMed ID: 16920798 |
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| Jenkins EC, Ye L, Gu H, Wisniewski HM, Mitotic index and Alzheimer's disease. Neuroreport9(17):3857-61 1998 |
| PubMed ID: 9875718 |
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| Martin ES, Martin SE, Edelsohn L, Borgaonkar DS, Studies in a large family with late-onset Alzheimer disease (LOAD). Alzheimer Dis Assoc Disord11:163-70 1997 |
| PubMed ID: 9305502 |
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| Borgaonkar DS, Schmidt LC, Martin SE, Kanzer MD, Edelsohn L, Growdon J, Farrer LA, Linkage of late-onset Alzheimer's disease with apolipoprotein E type 4 on chromosome 19 [letter] Lancet342:625 1993 |
| PubMed ID: 8102761 |
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| Borgaonkar, Studies in a family with late on-set familial Alzheimers disease. Am J Hum Genet51:A337 (1992):625 1992 |
| PubMed ID: 8102761 |
| Passage Frozen |
3 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Eagles Minimum Essential Medium with Earle's salts:Dulbecco's modified MEM with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Commercially-treated plastic |
| Supplement |
- |
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