GM03671
LCL from B-Lymphocyte
Description:
ACUTE LYMPHOCYTIC LEUKEMIA
LYMPHOSARCOMA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Heritable Cancer Syndromes and other Cancers |
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Biopsy Source
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Peripheral vein
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Cell Type
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T-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Possibly Human T-cell Leukemia Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
T cell line CCRF-CEM; virus particle free; positive for T cell 65,000 M.W. glycoprotein and negative for sIg and Ia (B cell) antigen; all cells show 8p-, -18, inv(X), and +20; 6% of cells are diploid, 94% tetraploid; all show random chromosome abnormalities |
| Goldman F, Bouarich R, Kulkarni S, Freeman S, Du HY, Harrington L, Mason PJ, Londoño-Vallejo A, Bessler M, The effect of TERC haploinsufficiency on the inheritance of telomere length Proceedings of the National Academy of Sciences of the United States of America102:17119-24 2005 |
| PubMed ID: 16284252 |
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| Knudson M, Kulkarni S, Ballas Z, Bessler M, Goldman F, Association of Immune Abnormalities with Telomere Shortening in Autosomal Dominant Dyskeratosis Congenita. BloodEpub ahead of print:17119-24 2004 |
| PubMed ID: 15238429 |
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| Lattier DL, States JC, Hutton JJ, Wiginton DA, Cell type-specific transcriptional regulation of the human adenosine deaminase gene. Nucleic Acids Res17:1061-76 1989 |
| PubMed ID: 2784203 |
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| Wellner VP, Anderson ME, Puri RN, Jensen GL, Meister A, Radioprotection by glutathione ester: transport of glutathione ester into human lymphoid cells and fibroblasts. Proc Natl Acad Sci U S A81:4732-5 1984 |
| PubMed ID: 6146978 |
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| Dalla-Favera R, Franchini G, Martinotti S, Wong-Staal F, Gallo RC, Croce CM, Chromosomal assignment of the human homologues of feline sarcoma virus and avian myeloblastosis virus onc genes. Proc Natl Acad Sci U S A79:4714-7 1982 |
| PubMed ID: 6289315 |
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| Daddona PE, Human adenosine deaminase. Properties and turnover in cultured T and B lymphoblasts. J Biol Chem256:12496-501 1981 |
| PubMed ID: 6975278 |
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| Latt SA, Barell EF, Dougherty CP, Lazarus H, Patterns of late replication in X chromosomes of human lymphoid cells. Cancer Genet Cytogenet3:171-81 1981 |
| PubMed ID: 6974041 |
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| Omary MB, Trowbridge IS, Covalent binding of fatty acid to the transferrin receptor in cultured human cells. J Biol Chem256:4715-8 1981 |
| PubMed ID: 6262300 |
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| Pollack MS, Heagney SD, Livingston PO, Fogh J, HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J Natl Cancer Inst66:1003-12 1981 |
| PubMed ID: 7017212 |
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| Ralston MD, DeMartino JL, Howe RC, Humphreys RE, Identification of external membrane proteins of the T lymphoblast cell line CCRF-CEM. Exp Cell Res123:237-45 1979 |
| PubMed ID: 315324 |
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| Carrel S, Gross N, Salvi S, Rabbit antiserum against Daudi-cell membrane fractions detects cell surface antigens present on subpopulations of human lymphocytes. J Immunol120:1587-93 1978 |
| PubMed ID: 307019 |
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| Kaplan J, Shope TC, Peterson WD Jr, Epstein-barr virus-negative human malignant T-cell lines. J Exp Med139:1070-6 1974 |
| PubMed ID: 4363409 |
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| Royston I, Graze PR, Pitts RB, Failure of cultured human T-cell lymphoid lines to stimulate in mixed leukocyte culture. J Natl Cancer Inst53:361-7 1974 |
| PubMed ID: 4276467 |
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| Krishan A, Raychaudhuri R, Flowers A, Karyotype studies on human leukemic lymphoblasts in vitro and as serial transplants in neonatal syrian hamsters. J Natl Cancer Inst43:1203-14 1969 |
| PubMed ID: 5273072 |
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| Adams RA, Foley GE, Uzman BG, Farber S, Lazarus S, Kleinman L, Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res27:772-83 1967 |
| PubMed ID: 4295047 |
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| Uzman BG, Foley GE, Farber S, Lazarus H, Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer19:1725-42 1966 |
| PubMed ID: 5224274 |
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| Foley, Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer18:522 (1965):1725-42 1965 |
| PubMed ID: 5224274 |
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| McCarthy RE, Junius V, Farber S, Lazarus H, Foley GE, Cytogenetic analysis of human lymphoblasts in continuous culture. Exp Cell Res40:197-200 1965 |
| PubMed ID: 5320250 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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