Description:
ISOCHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XX,i(13)(qter>q10::q10>qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Cytogenetics |
Chromosome 13: ISOCHROMOSOME Aneuploid Segment (+)13q10>13qter |
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Chromosome 13: ISOCHROMOSOME Aneuploid Segment (-)13p10>13pter |
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Chromosome 13: ISOCHROMOSOME Trisomic Segment 13q10>13qter |
| Remarks |
Cleft lip & palate; empty right eye socket; radial denervation of wrists; duplication of great toes; scalp hemangioma; passage 3 at IMR; 46,XX,r(13) was also seen in blood lymphocytes |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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