NA20259
DNA from Tumor-derived cell line
Description:
TRANSLOCATED CHROMOSOME
NON-HODGKINS LYMPHOMA
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Chromosome Abnormalities
dbGaP |
| Quantity |
50 µg |
| Quantitation Method |
Please see our FAQ |
|
Cell Type
|
Tumor-derived cell line
|
|
Tissue Type
|
Tumor
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
DNA from Tumor-derived cell line
|
|
Race
|
White
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
48~50,XXYY,t(1;14)(q21;q32),der(2)t(2;3;10),der(6)t(6;16;18),der(7)t(7;15),del(8)(p?),-10,dup(11),+13,der(15)t(15;18)(p11.2;q11.2),der(17)t(8;17)(q13;q25),-18,+2~7mar[cp25]
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Cytogenetics |
Chromosome 1: TRANSLOCATION Breakpoint 1q21 |
|
Chromosome 14: TRANSLOCATION Breakpoint 14q32 |
| Remarks |
Cell line SKI-DLCL-1 was established from a donor diagnosed with recurrent mantle cell lymphoma; ascitic fluid was removed from the donor and mononuclear cells were seeded at various cell concentrations in six-well plates. After four weeks the cells started to grow in suspension. This line had cells identical in morphology to the cells isolated at diagnosis. These cells have a high nuclear-cytoplasmic ratio with prominent nucleoli and numerous vacuoles. They demonstrate a profile of a mature B-cell phenotype identical to the original tumor: sIgM+, CD45+, CD10+, CD19+, CD20+, CD23+, CD3-, and CD5-; This cell line will only be shipped frozen. . |
| Goy A, Gilles F, Remache Y, Filippa D, Portlock CS, Jhanwar SC, Zelenetz AD, Establishment of a human cell line (SKI-DLCL-1) with a t(1;14)(q21;q32) translocation from the ascites of a patient with diffuse large cell lymphoma Leukemia & lymphoma40:419-23 2001 |
| PubMed ID: 11426565 |
|
|