NG06872
DNA from Fibroblast
Description:
TRISOMY 21
NIA AGING CELL REPOSITORY DNA PANEL - DOWN SYNDROME
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Chromosome Abnormalities
Heritable Diseases |
| Quantity |
10 µg |
| Quantitation Method |
Please see our FAQ |
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Biopsy Source
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Thorax/abdomen
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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DNA from Fibroblast
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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47,XX,+21[17]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
6.43 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The donor had typical features of Down syndrome (trisomy 21). The skin biopsy was taken post-mortem on 5/19/83. The culture was initiated using explants of minced skin tissue. The karyotype is 47,XX,+21. The cell morphology is fibroblast-like. Same subject as AG28278 (iPSC). The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Jin M, Ma Z, Jiang P, Generation of iPSC-based human-mouse microglial brain chimeras to study senescence of human microglia STAR protocols3:101847 2022 |
| PubMed ID: 36595906 |
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| Ahmed AA1, Smoczer C1, Pace B1, Patterson D2,3, Cress Cabelof D1., Loss of DNA polymerase β induces cellular senescence Environmental and Molecular Mutagenesis
3:101847 2018 |
| PubMed ID: 29968395 |
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| Deyle DR, Li LB, Ren G, Russell DW, The effects of polymorphisms on human gene targeting Nucleic Acids Res3:101847 2013 |
| PubMed ID: 24371280 |
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| Malchenko S, Xie J, de Fatima Bonaldo M, Vanin EF, Bhattacharyya B, Galat V, Goossens W, Seftor RE, Crispino J, Miller R, Bohn MC, Hendrix MJ, Soares MB., Onset of rosette formation during spontaneous neural differentiation of hESC and hiPSC colonies. Gene(http://www.sciencedirect.com/science/article/pii/:101847 2013 |
| PubMed ID: 23954875 |
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| Prandini P, Deutsch S, Lyle R, Gagnebin M, Vivier CD, Delorenzi M, Gehrig C, Descombes P, Sherman S, Bricarelli FD, Baldo C, Novelli A, Dallapiccola B, Antonarakis SE, Natural gene-expression variation in down syndrome modulates the outcome of gene-dosage imbalance American journal of human genetics81:252-63 2007 |
| PubMed ID: 17668376 |
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| Li CM, Guo M, Salas M, Schupf N, Silverman W, Zigman WB, Husain S, Warburton D, Thaker H, Tycko B, Cell type-specific over-expression of chromosome 21 genes in fibroblasts and fetal hearts with trisomy 21 BMC medical genetics [electronic resource]7:24 2005 |
| PubMed ID: 16539728 |
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