CH00096

CH00096 Neuronally-derived from Brain striatum

Description:

ST HDH Q7/111
HUNTINGTON DISEASE; HD

Affected:

No Data

Sex:

No Data

Age:

No Data

Overview

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Repository

HD Community Biorepository

Biopsy Source

Brain striatum

Cell Type

Neuronally-derived

Tissue Type

Brain

Transformant

Untransformed

Sample Source

Neuronally-derived from Brain striatum

Species

Mus musculus

Common Name

house mouse

Remarks

striatal derived cell line from a knock in transgenic mouse containing heterozygous Huntingtin (HTT) loci with a humanized Exon 1 with 111 polyglutamine repeats and the other with a humanized Exon 1 with 7 polyglutamine repeats **Please be advised that Dr. M. MacDonald recommends keeping the passage number of the cells below P14** CELLS SHIPPED FROZEN

Characterizations

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Remarks

striatal derived cell line from a knock in transgenic mouse containing heterozygous Huntingtin (HTT) loci with a humanized Exon 1 with 111 polyglutamine repeats and the other with a humanized Exon 1 with 7 polyglutamine repeats **Please be advised that Dr. M. MacDonald recommends keeping the passage number of the cells below P14** CELLS SHIPPED FROZEN

PDL at Freeze

5.77

Passage Frozen

12

Phenotypic Data

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Remarks

striatal derived cell line from a knock in transgenic mouse containing heterozygous Huntingtin (HTT) loci with a humanized Exon 1 with 111 polyglutamine repeats and the other with a humanized Exon 1 with 7 polyglutamine repeats **Please be advised that Dr. M. MacDonald recommends keeping the passage number of the cells below P14** CELLS SHIPPED FROZEN

Publications

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Trettel F, Rigamonti D, Hilditch-Maguire P, Wheeler VC, Sharp AH, Persichetti F, Cattaneo E, MacDonald ME, Dominant phenotypes produced by the HD mutation in STHdh(Q111) striatal cells Human molecular genetics9:2799-809 2000

PubMed ID: 11092756

External Links

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Gene Cards

Gene Ontology

GO:0003714 transcription corepressor activity

GO:0005215 transporter activity

GO:0005515 protein binding

GO:0005625 soluble fraction

GO:0005634 nucleus

GO:0005737 cytoplasm

GO:0006915 apoptosis

GO:0006917 induction of apoptosis

GO:0007610 behavior

GO:0008017 microtubule binding

GO:0009405 pathogenesis

GO:0009887 organogenesis

NCBI Gene

NCBI GTR

143100 HUNTINGTON DISEASE; HD

OMIM

143100 HUNTINGTON DISEASE; HD

Culture Protocols

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striatal derived cell line from a knock in transgenic mouse containing heterozygous Huntingtin (HTT) loci with a humanized Exon 1 with 111 polyglutamine repeats and the other with a humanized Exon 1 with 7 polyglutamine repeats **Please be advised that Dr. M. MacDonald recommends keeping the passage number of the cells below P14** CELLS SHIPPED FROZEN

Passage Frozen

12

Split Ratio

1:6

Temperature

33 C

Percent CO2

5%

Percent O2

AMBIENT

Medium

Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent

Serum

10% fetal bovine serum Not inactivated

Remarks

striatal derived cell line from a knock in transgenic mouse containing heterozygous Huntingtin (HTT) loci with a humanized Exon 1 with 111 polyglutamine repeats and the other with a humanized Exon 1 with 7 polyglutamine repeats **Please be advised that Dr. M. MacDonald recommends keeping the passage number of the cells below P14** CELLS SHIPPED FROZEN

PDF

CH00096 Cell Culturing Protocols

Supplement

geneticin/G418 400 ug/ml

Pricing

Commercial:

$100.00USD

Academic &
Non-profit:

$100.00USD