AG06239
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
34 |
| PDL at Freeze |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XY; diploid male. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Emmert S, Slor H, Busch DB, Batko S, Albert RB, Coleman D, Khan SG, Abu-Libdeh B, DiGiovanna JJ, Cunningham BB, Lee MM, Crollick J, Inui H, Ueda T, Hedayati M, Grossman L, Shahlavi T, Cleaver JE, Kraemer KH, Relationship of neurologic degeneration to genotype in three xeroderma pigmentosum group g patients. J Invest Dermatol118(6):972-82 2002 |
| PubMed ID: 12060391 |
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| Khan SG, Muniz-Medina V, Shahlavi T, Baker CC, Inui H, Ueda T, Emmert S, Schneider TD, Kraemer KH, The human XPC DNA repair gene: arrangement, splice site information content and influence of a single nucleotide polymorphism in a splice acceptor site on alternative splicing and function. Nucleic Acids Res30(16):3624-31 2002 |
| PubMed ID: 12177305 |
| PDL at Senescence |
34 |
| Cumulative PDL at Freeze |
8 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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