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NA11023 DNA from Somatic cell hybrid

Description:

CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Quantity 0.050mg
Quantitation Method Please see our FAQ
Cell Type Somatic cell hybrid
Transformant Untransformed
Sample Source DNA from Somatic cell hybrid
Confirmation Karyotypic analysis and In situ hybridization
ISCN Human/mouse hybrid retaining human der (X)t(X;4), #3 through 8, 10, 11, & 14 through 22
Remarks Line IC-C3; produced by fusing human lymphoblasts, GM11025, 46,XX,t(X;4)(p21; q35), with HPRT-def, ouabain-resistant mouse RAG cells; selected in HAT medium; retains hum der(X)t(X;4) in 96% of cells & other human chromosomes including #4

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD, and gave a negative result with a primer for Xp22.32, STS.
 
GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, 2q13, IL1A, and 2q37, ALPP.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 4p15.3, QDPR, and 4q31-q33, 4S1626.
 

Phenotypic Data

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Remarks Line IC-C3; produced by fusing human lymphoblasts, GM11025, 46,XX,t(X;4)(p21; q35), with HPRT-def, ouabain-resistant mouse RAG cells; selected in HAT medium; retains hum der(X)t(X;4) in 96% of cells & other human chromosomes including #4

Publications

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Harun RB, Smith KK, Leek JP, Markham AF, Norris A, Morrison JF, Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1. Genomics42:349-52 1997
PubMed ID: 9192859
 
Mills KA, Buetow KH, Xu Y, Ritty TM, Mathews KD, Bodrug SE, Wijmenga C, Balazs I, Murray JC, Genetic and physical mapping on chromosome 4 narrows the localization of the gene for facioscapulohumeral muscular dystrophy (FSHD). Am J Hum Genet51:432-9 1992
PubMed ID: 1642243

External Links

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dbSNP dbSNP ID: 11474
Pricing
International/Commercial/For-profit:
$449.00USD
U.S. Academic/Non-profit/Government:
$263.00USD
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