Induced Pluripotent Stem Cells

To facilitate research on differentiated cell types and other biomedical investigations, the NIGMS Repository offers highly characterized human induced pluripotent stem cell (hiPSC) lines derived from apparently healthy individuals as well as from individuals affected with heritable diseases. These hiPSCs were created using a variety of reprogramming techniques* and are suitable for use in cell differentiation, disease models, drug discovery, genotyping, and as experimental controls. Additionally, gene-edited lines have been generated using CRISPR Cas9 gene-editing technology* from apparently healthy and patient-derived disease specific iPSCs.

We have developed rigorous scientific practices to maintain, characterize, bank, and distribute only the highest quality iPSCs available. Our laboratories perform the most stringent quality control testing on all samples, including:

  • Viability
  • Sterility
  • Karyotyping
  • Microsatellite matches with the parent cell culture
  • Extensive pluripotency characterization
  • Surface antigen expression
  • Embryoid body formation

A complete Certificate of Analysis (CofA) specifies pluripotency characterizations that were performed for each hiPSC, including images and quantitative test results.

Available with each line is a protocol which outlines the proper steps for successful handling of the hiPSC. Additional information is also found on the CofA, including necessary media components, substrates, passage methods, and split ratios.

Click on the images below to access the catalog for hiPSCs available from the NIGMS Repository

   Apparently Healthy iPSCs   Disease-Specific iPSCs  Gene-Edited iPSCs

*For commercial purposes, please note that Limited Use Label Licenses (LULLs) may apply.